Analysis of the NAC Gene Family in Salix and the Identification of SpsNAC005 Gene Contributing to Salt and Drought Tolerance

The NAC gene family is of great value for plant stress resistance and development. In this study, five genes with a typical NAM domain were isolated from Salix psammophila, which stress-resistant willow endemic to western China. Two hundred sixty-two purpurea, Arabidopsis used construct the phylogenetic tree examine relationship. Five in psammophila focus bioinformatics analysis conserved structural analysis. SpsNAC005 was overexpressed Populus hopeiensis, transgenic lines subjected salt simulated drought analyze their phenotype changes tolerance stress. results showed that poplar height leaf area increased by 29.73% 76.36%, respectively, compared those wild-type plants. Under treatment, growth rates ground diameter significantly higher than wild-type, whereas fresh weight dry decreased wild-type. SOD activities, POD Pro contents plants increased, accumulation MDA lower clear drought. expressions SOS1, MPK6, HKT1, P5CS1 downregulated lines. expression PRODH1 These indicate overexpression can promote development improve